Kelly A. Daggett Kelly A. Daggett, Postdoctoral Associate
Laboratory of Molecular Biology and Biochemistry
Rockefeller Research Building 510

The Rockefeller University, Box 187
1230 York Avenue
New York, NY 10065

Telephone: 212-327-8284
Fax: 212-327-7904

daggettk@mail.rockefeller.edu


pdfCurriculum Vitae




Education:
Postdoctoral Researcher, Rockefeller University, 2010 - present
Ph.D. in Chemistry, University of Maryland, College Park, 2004-2009
B.S. in Biochemistry, Manhattan College, 2000-2004



Research:
During my graduate career I became very interested in the genetic incorporation of unnatural amino acids. I had worked on the development of an isotopically labled p-benzoyl phenylalanine for mass spectral studies. When incorporated in a 50/50 mix with unlabeled results in a unique fingerprint of M and M+11 and allows for the rapid identification of interacting proteins. This work then sparked the question: What if there is no previous knowledge of the interacting surface? How can this probe be inserted at every position? This question was addressed by developing a method that uses simple molecular biology techniques that facilitates the random incorporation of a specific codon, all without the use of oligonucleotides. Since I have joined the Sakmar lab, I now get to further my knowledge of the genetic incorporation unnatural amino acids and apply methodologies to answer questions about GPCR signaling. I will be utilizing the unique photo crosslinking amino acid p-benzoyl-L-phenylalanine to identify and characterize interactions occurring with receptors.

Awards:
Distinguished Teaching Assistant, University of Maryland, College Park, 2007 - 2008
GAANN Teaching Fellow, University of Maryland, College Park, 2006 -2007
Chemistry Honors Medal, Manhattan College, 2004
Sigma Xi, Research Honor Society, Manhattan College, 2004
Howard Hughes Medical Grant, Manhattan College, Summer 2003
Gamma Sigma Epsilon, Chemistry Honor Society, Manhattan College, 2003



Published Papers:
1).
Kelly A. Daggett, Mark Layer and T. Ashton Cropp
A General Method for Scanning Unnatural Amino Acid Mutagenesis
ACS Chem. Biol. 2009, 4, 109

2).
Bryan J. Wilkins, Kelly A. Daggett and T. Ashton Cropp
Peptide Mass Fingerprinting Using Isotopically Encoded Photo-crosslinking
Amino Acids
Mol. Biosyst. 2008, 4, 934.

3).
James V. McCullagh and Kelly A. Daggett
Synthesis of Triarylmethane and Xanthene Dyes Using Electrophilic Aromatic
Substitution Reactions
J. Chem. Educ. 2007, 84, 1799



Recent Presentations:
Daggett, K.A.; Cropp, T.A.
“A General Method for Scanning Photoreactive Amino Acids”
238th ACS National Meeting, Washington DC. August 2009.

Daggett, K.A.; Cropp, T.A.
“A General Method for Single-Residue Scanning Protein Mutagenesis”
National Cancer Institute, Chemistry of Biological Processes Symposium, Frederick, MD. August 2008.

Daggett, K.A. and Cropp, T.A.
“Codon Scanning Mutagenesis: A general method for single-residue scanning protein mutagenesis”
Maryland Interface of Chemistry and Biology Meeting, College Park, MD. April 2008.

Daggett, K.A.; Wilkins, B.J. and Cropp, T.A.
“A Novel Random Mutagenesis Method and the Incorporation of an Isotopic Label into a Protein”
Experimental Biology Annual Meeting,
Washington DC. April 2007.

Daggett, K.A.; Wilkins, B.J.; Cropp, T.A.
“A Novel Method for Scanning Unnatural Amino Acid Mutagenesis”
Reaction Mechanisms Conference,
College Park, MD. June 2006.

Daggett, K.A.; McCullagh J.
“Demonstration of Electrophilic Aromatic Substitution Reactions in an Organic Lab Experiment: Synthesis of Triaryl Methine Dyes”
American Chemical Society National Meeting,
Anaheim, CA.March 2004.

Daggett, K; DeThomas EM; Kerrigan PK; Haley J.
“Small Scale Synthesis, Purification and Characterization of Meso Substituted Porphyrins”
American Chemical Society National Meeting,
New York City, NY.  September 2003.



Posters: