Kelly A. Daggett, Postdoctoral Associate Laboratory of Molecular Biology and Biochemistry Rockefeller Research Building 510 The Rockefeller University, Box 187 Telephone: 212-327-8284 Curriculum Vitae |
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Education: |
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Research: During my graduate career I became very interested in the genetic incorporation of unnatural amino acids. I had worked on the development of an isotopically labled p-benzoyl phenylalanine for mass spectral studies. When incorporated in a 50/50 mix with unlabeled results in a unique fingerprint of M and M+11 and allows for the rapid identification of interacting proteins. This work then sparked the question: What if there is no previous knowledge of the interacting surface? How can this probe be inserted at every position? This question was addressed by developing a method that uses simple molecular biology techniques that facilitates the random incorporation of a specific codon, all without the use of oligonucleotides. Since I have joined the Sakmar lab, I now get to further my knowledge of the genetic incorporation unnatural amino acids and apply methodologies to answer questions about GPCR signaling. I will be utilizing the unique photo crosslinking amino acid p-benzoyl-L-phenylalanine to identify and characterize interactions occurring with receptors. |
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Awards: |
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Published Papers: 2). 3). |
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Recent Presentations: Daggett, K.A.; Cropp, T.A. Daggett, K.A. and Cropp, T.A. Daggett, K.A.; Wilkins, B.J. and Cropp, T.A. Daggett, K.A.; Wilkins, B.J.; Cropp, T.A. Daggett, K.A.; McCullagh J. “Small Scale Synthesis, Purification and Characterization of Meso Substituted Porphyrins” American Chemical Society National Meeting, New York City, NY. September 2003. |
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Posters: |